Friday success / failure thread

Been selecting my namesake for tissue culturability / transformability. I've had only two plates out of about 90 get contaminated. Chinese guy who knows a lot about transformability of maize has led me to believe that some of the stuff I had as Type-II callus but is no longer Type-II after ~2 months may have been good, possibly making a more rapid cycling transformation process. I'm not sure because of language barriers and because I think it would make it a more one-shot deal; if you can't get the regenerated plants to pollinate you're screwed because you don't have back-up callus. I have been selecting for embryos that remained as Type-II callus for a long time, which is a trait of the line people use for maize transformation. I figured anything that made it 2+ months and still made good callus was what I wanted, so I didn't bother taking notes on each of the thousands of embryos that have been whittled down to ~ 80-90 plates of 3-12+ conglomerates of cells. Also this is not my thesis project and I'm already spending an inordinate amount of time on it.

Turned a bunch of data I had represented as box-plots into tables, working on consolidating all the box-plots into one multi-part figure for the paper, or for the supplementary data. Otherwise I have everything else in the format that Genetics wants. Other than one thing, I cannot figure out how to label one figure with parts A-F in Photoshop using MS Sans Serif BOLD. I can get Sans Serif, but there is no style option in Photoshop for bold. I googled it and there are a ton of people with the same problem as me and no solution. I can't install anything on our computers, admins have to do it, so I'm SOL for a few days.

For the holidays I bought a bottle of Laphroaig and a turkey, so I'm going to cook my first turkey. I have a few turkey protocols (recipes) and I think I know what I'm getting into.

/r/labrats Thread