Very challenging, unless you can create a narrative that connects why you decided to research this new topic that is unrelated to methodology.

Example:

A. In grad school you research whether or not emotion is related to donation behavior. (Simplistic, I know, but it's just an example!) You do this using neuroimaging. But you then do a totally unrelated project on whether or not past trauma predicts poor outcomes in romantic relationships, also using neuroimaging.

B. In grad school, you research whether or not emotion is related to donation behavior using neuroimaging. You also do a project on whether or not physiological arousal is related to donation behavior, this time using psychophysiological measures.

C. In grad school, you research whether or not emotion is related to donation behavior using neuroimaging. You also do a project on whether donation behavior is associated with prejudice using implicit behavioral measures.

D. In grad school, you research whether or not emotion is related to donation behavior using neuroimaging. You also research whether or not emotion predicts prejudice using neuroimaging.

B/C/D, you can justify as being part of a cohesive research program. A is nonsensical -- the only thing there is in common here is the measure. You might be able to create a narrative somehow connecting emotion and donation behavior with trauma and romantic relationships, but you'd be fighting an uphill battle.

The exception is if the measure is something so specific that you're one of the only people in the field who knows anything about it and there's some kind of theoretical underpinning unique to the measure. This is uncommon.

I had this problem coming out of undergrad where I'd done a lot of physiological and implicit work, but in a different subfield than I wanted to go into. I was able to frame it in my grad school applications as being interested in the component processes of emotion (all of my projects had to do with some kind of emotion or emotion dysregulation). That's an example of where you can make it work for you, but that's a lot easier to do in undergrad-to-grad school where you aren't expected to have it all figured out than it is in grad school-to-professorship where you're expected to have a brand.

This may be totally different though for industry jobs, which I know nothing about, so ymmv.